An rbcL sequence from a Miocene Taxodium (bald cypress).

نویسندگان

  • P S Soltis
  • D E Soltis
  • C J Smiley
چکیده

During the past decade, ancient DNAs from both animals and plants have been successfully extracted and analyzed. Recently, the age of DNA that can be recovered and sequenced was increased manyfold by the amplification and sequencing of a DNA fragment from a Magnolia fossil obtained from the Miocene Clarkia deposit (17-20 million yr old). However, the validity of this report has been questioned based on models predicting that DNA should be completely degraded after 4 million yr. We report here the successful amplification, sequencing, and analysis of a 1320-base-pair portion of the chloroplast gene rbcL from a Miocene Taxodium specimen, also from the Clarkia site. These data not only validate the earlier report of sequence data for a Magnolia species from the same site but also suggest that it may be possible to isolate and sequence DNAs routinely from the Clarkia deposit. The ability to recover and sequence DNAs of such age offers enormous research possibilities in the areas of molecular evolution, biogeography, and systematics.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of Long-Term Periodic Submergence on Photosynthesis and Growth of Taxodium distichum and Taxodium ascendens Saplings in the Hydro-Fluctuation Zone of the Three Gorges Reservoir of China

Responses of bald cypress (Taxodium distichum) and pond cypress (Taxodium ascendens) saplings in photosynthesis and growth to long-term periodic submergence in situ in the hydro-fluctuation zone of the Three Gorges Dam Reservoir (TGDR) were studied. Water treatments of periodic deep submergence (DS) and moderate submergence (MS) in situ were imposed on 2-year-old bald cypress and pond cypress s...

متن کامل

Endophytic taxol-producing fungi from bald cypress, Taxodium distichum.

Pestalotiopsis microspora occurs as a range of strains in bald cypress, Taxodium distichum. The organisms live as endophytes in the bark, phloem and xylem, and isolates show differences in cultural and microscopic characteristics on common laboratory media. Many of these fungi make taxol as determined by the reactivity of partially purified culture extracts with specific monoclonal antibodies a...

متن کامل

Seed germination enhancement for bald cypress [Taxodium distichum (L.) Rich.]

Bald cypress has been retreating in the estuary ecosystem of the Loxahatchee River Watershed in southeastern Florida because of the negative impacts of anthropogenic activities. Seed germination of bald cypress is an important means to increase the arbor population in the vegetation community and accelerate restoration of the wetland ecosystem. The objective of this research was to find an effe...

متن کامل

Inner Workings: How saving some of the Southeast's oldest trees might help scientists monitor climate change.

In the swampy floodplains of North Carolina’s Black River stand ancient trees with a story to tell. Many of these bald cypress (Taxodium distichum) were well over a millennium old when Columbus discovered the Western Hemisphere. As it turns out, their locale has greatly promoted their longevity: by being relatively inaccessible, the trees have been spared from loggers’ saws. But the inexorable ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 89 1  شماره 

صفحات  -

تاریخ انتشار 1992